Journal: Immunity

Article Title: Differentiation and protective capacity of virus-specific CD8 + T cells suggest murine norovirus persistence in an immune-privileged enteric niche

doi: 10.1016/j.immuni.2017.09.017

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Dead cells were removed by gating on a LIVE/DEAD Aqua kit (Invitrogen, Carlsbad, CA) versus forward scatter (FSC-H). table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti CD8β-PerCP-Cy5.5 (clone YTS156.7.7) Biolegend Cat# 126609 Anti PD-1-PE-Cy7 (clone RMP1-30) Biolegend Cat# 109109 Anti CD103-PacificBlue (clone 2E7) Biolegend Cat# 121417 Anti CD44-BV785 (clone IM7) Biolegend Cat# 103041 Anti CD45.2-BV785 (clone 104) Biolegend Cat# 109839 Anti CXCR4-BV421(clone L276F12) Biolegend Cat# 146511 Anti Ly6c-BV785 (clone HK1.4) Biolegend Cat# 128041 Anti KLRG-1-BV605 (clone 2F1) Biolegend Cat# 138419 Anti CD62L-BV605 (clone MEI-14) Biolegend Cat# 104438 Anti TNFa-PacificBlue (clone MP6-XT22) Biolegend Cat# 506318 Anti CD107a-AF488 (clone 1D4B) Biolegend Cat# 121607 Anti CD28-PE-Cy7 (clone 37.51) Biolegend Cat# 102126 Anti CX3CR1-PE (clone SA011F11) Biolegend Cat# 149006 Anti CD11a-PE (clone 2D7) Biolegend Cat# 101008 Anti FOXP3-PE (clone MF-14) Biolegend Cat# 126404 Anti CD90.1 (Thy-1.1)-BV605 (clone OX-7) Biolegend Cat# 202537 Anti CD25-PE-Cy5 (clone PC61) Biolegend Cat# 102010 Anti CD8a-PE-eF610 (clone 53.6) eBioscience Cat# 61-0081-80 Anti CD69-PE-Cy7 (clone H1.2F3) eBioscience Cat# 25-0691-82 Anti 2B4-FITC (clone eBio244F4) eBioscience Cat# 11-2441-82 Anti CD49b-PE-Cy5 (clone DX5) eBioscience Cat# 15-5971-82 Anti CD49d-FITC (clone R1-2) eBioscience Cat# 11-0492-81 Anti CD90.2 (Thy1.2)-FITC (clone 30-H12) eBioscience Cat# 11-0903-82 Anti CD27-APC-eFluor 780 (clone LG.7F9) eBioscience Cat# 47-0271-82 Anti CD45.1-APC-R700 (clone A20) BD Biosciences Cat# 565814 Anti IFN -AlexaFluor700 (clone XMG1.2) BD Biosciences Cat# 557998 Anti GITR-FITC (clone DTA-1) BD Biosciences Cat# 558139 Anti Granzyme-B-PE (clone GB11) Invitrogen Cat# GRB04 Anti MIP-1a-APC (clone 39624) R&D Systems Cat# 1C450A Anti IL-12Rβ2-PRCP (clone 305719) R&D Systems Cat# FAB1959C Anti-CD8β (Lyt 3.2) (clone 53-5.8) Bio X Cell Cat # BE0223 Anti-Thy1.1 (clone 19E12) Bio X Cell Cat # BE0214 Bacterial and Virus Strains MNV-CW3 Tomov et al., 2013 GenBank EF014462 MNV-CR6 Tomov et al., 2013 GenBank EU004676 MNV-CW3 Y→F This study N/A MNV-CR6 F→Y This study N/A rVV 519Y This study N/A Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Deoxyribonuclease I from Bovine Pancreas Sigma-Aldrich Cat # D5025 Collagenase/Dispase Roche Cat # 11097113001 DTT (dithiothreitol) Thermo Fisher Cat # R0861 RPMI 1640 Corning Cat # 15-040-CM GemCell U.S.

Techniques: Virus, Recombinant, Saline, Reverse Transcription, Gene Expression, SYBR Green Assay, Isolation, Staining, Microarray, Plasmid Preparation, Software